Sep 07, 2020 · Fill vials with sample solution and place them into the sample tray. Vial position is 1:A,2. Select the pump A, Solvent Line A1, or A2. Inject 5µl of sample solution (25ppm Caffeine in water) at 0.6, 0.4, 0.2 & 0.1 ml/min flow rate. Set the run time 2.0 min, 2.0 min, 5.0 min and 10.0 min, respectively for above mentioned flow rate.