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Once phytohormones were identified and the SPE protocol optimized, phytohormones from the rice samples collected and listed in Table 1 were extracted using three independent replicates, using an internal deuterium-labeled standard for each phytohormone, with the method C’ (explained below) and quantified in an HPLC-QqQ instrument with a method called HPLC-2 described in Table 2.

Jun 07, 2019 · The HPLC column Kinetex ® 2.6 μm XB-C18 100 Å (30 2.1 mm) was purchased from Phenomenex (Torrance, CA, United States), and the HPLC column Mediterranea Sea 18 column (10 0.2 cm, 2.2 μm) was purchased from Teknokroma (Sant Cugat del Vallès, Spain).

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An experimental feasibility study on continuous bioprocessing in pilot-scale of 1 L/day cell supernatant, that is, about 150 g/year product (monoclonal antibody) based on CHO (Chinese hamster ovary) cells for model validation is performed for about six weeks including preparation, start-up, batch, and continuous steady-state operation for at least two weeks stable operation as well as final

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