pH of 8- 10, but also contains 20-29% sulfonate in the anhydrous form.29 Sigma Aldrich,. (St. Louis, MO) sells a “low sulfonate alkali (kraft) lignin”
This chapter describes the standard method to process gDNA prior to labeling as well as the Whole Genome Amplification method for low input DNA projects. 4
A small volume (150 μL) of serum/plasma is mixed with an equal volume of buffer, and then mixed Retinyl Palmitate (Sigma-Aldrich, St. Louis, Missouri).
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For our organoid protocols, we use Growth Factor Reduced Matrigel that is smaller opening through which organoids, Matrigel, and medium are pipetted.
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In two colorectal adenocarcinoma cell lines, high levels of AQP1 transcript were confirmed in HT29, and low levels in SW480 cells, by quantitative PCR (
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